snap-cap spin column scsc  (Fisher Scientific)

Journal: RSC Advances

Article Title: Quantitative analysis of fucosylated glycoproteins by immobilized lectin-affinity fluorescent labeling †

doi: 10.1039/d3ra00072a

Figure Lengend Snippet: Schematic diagram of lectin affinity fluorescent labeling quantification (LAFLQ) for quantitative determination of protein fucosylation. Proteins extracted from saliva are labeled with Alexa Fluor™ 488 (AF488), whose NHS ester reacts with N -terminus or lysine of proteins/glycoproteins. After quenching AF488 active NHS ester groups, the labeled proteins/glycoproteins were incubated with the lectin-beads. Glycoproteins bind to the beads via lectin affinity and proteins remain in solution. Unbound proteins or glycoproteins were removed by centrifugation in a SCSC. Glycoprotein-bound beads were loaded to a 96-well plate for further testing by a fluorescence detector. The relative abundance of glycoproteins is characterized by fluorescent intensity.

Article Snippet: AminoLinkTM plus resins (beads) and snap-cap spin column (SCSC) were purchased from Thermo Scientific (Waltham, MA, USA).

Techniques: Labeling, Incubation, Centrifugation, Fluorescence